2012-03-09
1997-01-17
See the manual for the WarmStart LAMP Kit (DNA & RNA), NEB #E1700, for more details. We suggest adding 10 ng Tte UvrD Helicase to a 25 μl LAMP reaction using the WarmStart ® LAMP Kit (NEB #E1700). Helicase should be added to all reactions, as it has minimal effect on performance of reactions containing the target DNA or RNA (though fluorescence signal may decrease; reaction time should not be affected). Tte-UvrD Helicase - 50 rxns: Amazon.com: Industrial & Scientific.
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Learn how Helicase-dependent Amplification enables primer annealing and extension by a strand-displacing DNA polymerase. UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke Cell. 2006 Dec 29;127(7):1349-60. doi: 10.1016/j.cell.2006.10.049.
2019-08-13
EcoRI UvrD/REP helicase N-terminal domain Provide feedback REP helicases catalyse ATP dependent unwinding of double stranded DNA to single stranded DNA. A helicase with DNA-dependent ATPase activity (PubMed:8419285). Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand.
transcriptase in presence of UvrD helicase that unwinds the RNA-DNA hybrid enabling 6.6µM Helicase (BioHelix), 2.5µM Template, 7.5µM ET SSB (NEB).
Helicase-dependent amplification (HDA) is an isothermal in vitro DNA amplification method based upon the coordinated actions of helicases to separate double-stranded DNA and DNA polymerases to synthesize DNA. Previously, a mesophilic form of HDA (mHDA) utilizing the Escherichia coli UvrD helicase… Tte Uvrd Helicase, supplied by New England Biolabs, used in various techniques.
Packaging Unit: 50 rxns. € 74,00.
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Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis. Tte UvrD Helicase Eliminates Non-Template Amplification in LAMP Reactions.
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1997-01-17 · Analysis of these kinetics indicates that UvrD unwinds duplex DNA in discrete steps, with an average "step size" of 4 to 5 base pairs (approximately one-half turn of the DNA helix). This suggests an unwinding mechanism in which alternating subunits of the dimeric helicase interact directly with duplex DNA.
Feb 24, 2014 The yeast Srs2 helicase removes Rad51 nucleoprotein filaments from single- stranded DNA (ssDNA), preventing DNA The crystal structure of SF-1 helicases such as UvrD, (Perkin Elmer) and polynucleotide kinase (NEB). The PcrA/UvrD helicase functions in multiple path- ways that promote bacterial (NEB) by incubating each reaction with beads taken from an equal volume of May 14, 1992 The role of the C terminus of Escherichia coli DNA helicase II (UvrD), Vent DNA polymerase were from NEB and used as recommended by NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies ®) · NEBNext® ARTIC Tte-UvrD Helicase · Exo-CIP Rapid PCR Cleanup Kit Tte UvrD Helicase (NEB #M1202).
We suggest adding 10 ng Tte UvrD Helicase to a 25 μl LAMP reaction using the WarmStart ® LAMP Kit (NEB #E1700). Helicase should be added to all reactions, as it has minimal effect on performance of reactions containing the target DNA or RNA (though fluorescence signal may decrease; reaction time should not be affected).
(A) RecA–ssDNA nucleoprotein filaments.
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